Infection by Grapevine fanleaf nepovirus (GFLV), a bipartite RNA virus of positive polarity belonging to the Comoviridae family, causes extensive cytopathic. The specific transmission of Grapevine fanleaf virus by its nematode vector Xiphinema index is solely determined by the viral coat protein. There are plenty of plant viruses that no one has heard of, but few are as widely known as grapevine fanleaf virus. Learn how to identify a sick.

Author: Faesho Vudokazahn
Country: Turkey
Language: English (Spanish)
Genre: Sex
Published (Last): 7 January 2007
Pages: 339
PDF File Size: 12.12 Mb
ePub File Size: 5.70 Mb
ISBN: 189-3-20387-402-5
Downloads: 62511
Price: Free* [*Free Regsitration Required]
Uploader: Dunos

These observations imply that GFLV replication depends both on ER-derived membrane recruitment and on de novo lipid synthesis.

Fanleaf Degeneration of Grape

Immunolabeling of viral proteins. No electron-dense structures similar to those described in CPMV-infected cells 15 were observed. Grapevine fanleaf nepovirus P38 putative movement protein is not transiently expressed and is a stable final maturation product in vivo.

By using this site, you agree to the Terms of Use and Privacy Policy. Protoplasts fixation and embedding for electron microscopy.

There was a problem providing the content you requested

In situ localization and in vitro induction of plant COPI-coated vesicles. The procedure was adapted from Bienz et al. The remaining protoplasts were fixed and immunolabeled with anti-VPg or anti-CP antibodies and A conjugate.

Open in a separate window. Unlike VPg grapsvine, the intracellular coat protein distribution was not homogeneous in between cells. Grapevine fanleaf nepovirus P38 putative movement protein is located on tubules in vivo. On the contrary, anti-VPg revealed several bands in total Fig. The virus has an RNA genome contained fanlaf two RNA molecules, each of which is covalently attached to a small protein the viral genome-linked protein, VPg at the 5′ end.


Canes are also malformed, showing abnormal branching, double nodes, different length or exceedingly short internodes, fasciations, and zigzag growth.

Grapevine Fanleaf Virus Replication Occurs on Endoplasmic Reticulum-Derived Membranes

Grapevine fanleaf degeneration disease is caused by the grapevine fanleaf virus GFLVa member of the nepovirus group. Photo courtesy of William M. Brefeldin A differentially affects protein secretion from suspension-cultured tobacco cells Nicotiana tabacum. Therefore, mock-electroporated and infected protoplasts harvested at 48 hpi were processed for immunolabeling with anti-CP or anti-VPg antibodies.

The type of membranes involved in replication depends grapveine the virus considered: To further refine the analysis of GFLV replication sites, the ultrastructural organization of the perinuclear aggregates containing the viral products was studied by electron microscopy on infected protoplasts Fig.

A human virus protein, poliovirus protein 2BC, induces membrane proliferation and blocks the exocytic pathway in the yeast Saccharomyces cerevisiae. Although some replication centers were found in the ER-rich perinuclear region, many of them remained peripheral.

Also, the productive life of GFLV-infected vineyards is significantly reduced.

Articles with ‘species’ microformats All stub articles. This phenomenon is particularly visible in Fig. The bound material was successively fixed with glutaraldehyde, tannic acid, and osmium tetroxide before observation under the electron microscope. The coat protein derives from RNA2 [5] and forms the icosahedral capsid of 60 identical protein subunits.

In contrast to proteins involved in viral replication, the 2B movement protein and, to a lesser extent, the 2C coat protein were not confined to the fanlear compartment but were transported toward the cell periphery, a finding consistent with their role in cell-to-cell movement of virus particles. No significant signal was observed in similarly processed healthy protoplasts Fig. Evidence for the involvement of virus-induced vesicles in poliovirus replication was obtained already in the sixties 10and yrapevine molecular mechanisms underlying the formation of virus-induced vesicles, their origin, and their role in viral replication were recently unraveled 1958 The fact that distinct types of membranes are involved in the replication of different viruses implies the establishment of specific interactions between such host membranes and virus-encoded proteins.


Because viral proteins accumulate to detectable levels only when replication takes place 23virhs percentage of fluorescent cells was considered to correspond to the percentage of infected cells between and 1, cells were counted per experiment. Effects of site-directed mutagenesis on the presumed catalytic triad and substrate-binding pocket of grapevine fanleaf nepovirus kDa proteinase.

The fixative was replaced by 2. Such rosettes were very reminiscent both in morphology and size to those isolated by ISEM from poliovirus-infected cells 45.

In hot climates the newly produced summer vegetation has a normal green color, while the yellowing of the old growth turns whitish. Try asking one of our Experts. Grapevine fanleaf virus symptoms – yellow mosaic pattern on leaf L and bright yellow vein banding on leaf. We are currently investigating the mechanism by which GFLV MP is transported intracellularly and whether it moves to the cell periphery alone or in association with virions.

Note in panels B and C the abundance of small vesicles arrows near the nucleus in infected cells and in panel C the presence of virus-like particles small arrows trapped in filamentous material star located at the periphery of the vesicular aggregates. The sedimentation of Golgi Fig.